Urinary 8-hydroxy-2′-deoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to fine particulates

Jae Young Kim, Sutapa Mukherjee, Long Ngo, David C. Christiani

Research output: Contribution to journalArticlepeer-review

125 Citations (Scopus)

Abstract

Residual oil fly ash (ROFA) is a chemically complex mixture of compounds, including metals that are potentially carcinogenic because of their ability to cause oxidative injury. In this study, we investigated the association between exposure to particulate matter with an aerodynamic mass median diameter ≤ 2.5 μm (PM 2.5 ) and oxidative DNA damage and repair, as indicated by urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) concentrations, in a group of boilermakers exposed to ROFA and metal fumes. Twenty workers (50% smokers) were monitored for 5 days during an overhaul of oil-fired boilers. The median occupational PM 2.5 8-hr time-weighted average was 0.44 mg/m 3 (25th-75th percentile, 0.29-0.76). The mean ± SE creatinine-adjusted 8-OHdG levels were 13.26 ± 1.04 μg/g in urine samples collected pre-workshift and 15.22 ± 0.99 μg/g in the post-workshift samples. The urinary 8-OHdG levels were significantly greater in the post-workshift samples than in the pre-workshift samples (p = 0.02), after adjusting for urinary cotinine levels, chronic bronchitis status, and age. Linear mixed models indicated a significant exposure-response association between PM 2.5 exposure and urinary 8-OHdG levels (p = 0.03). Each 1-mg/m 3 incremental increase in PM 2.5 exposure was associated with an increase of 1.67 μg/g (95% confidence interval, 0.21-3.14) in 8-OHdG levels. PM 2.5 vanadium, manganese, nickel, and lead exposures also were positively associated with 8-OHdG levels (p ≤ 0.05). This study suggests that a relatively young and healthy cohort of boilermakers may experience an increased risk of developing oxidative DNA injury after exposure to high levels of metal-containing particulate matter.

Original languageEnglish
Pages (from-to)666-671
Number of pages6
JournalEnvironmental Health Perspectives
Volume112
Issue number6
DOIs
Publication statusPublished - 1 May 2004
Externally publishedYes

Keywords

  • Biomarkers
  • Epidemiology
  • Occupational
  • Oxidative DNA damage
  • Particulate matter

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