Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

G B Rogers, M P Carroll, D J Serisier, P M Hockey, G Jones, V Kehagia, G J Connett, K D Bruce

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.
Original languageEnglish
Pages (from-to)2601-2604
Number of pages4
JournalJournal of Clinical Microbiology
Volume44
Issue number7
DOIs
Publication statusPublished - Jul 2006

Keywords

  • bacterial community
  • sputum samples
  • cystic fibrosis

Fingerprint Dive into the research topics of 'Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis'. Together they form a unique fingerprint.

  • Cite this