Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

G B Rogers; M P Carroll; D J Serisier; P M Hockey; G Jones; V Kehagia; G J Connett; K D Bruce

doi:10.1128/JCM.02282-05

Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

G B Rogers, M P Carroll, D J Serisier, P M Hockey, G Jones, V Kehagia, G J Connett, K D Bruce

College of Medicine and Public Health

Research output: Contribution to journal › Article › peer-review

139 Citations (Scopus)

Abstract

The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.

Original language	English
Pages (from-to)	2601-2604
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	7
DOIs	https://doi.org/10.1128/JCM.02282-05
Publication status	Published - Jul 2006

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

bacterial community
sputum samples
cystic fibrosis

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10.1128/JCM.02282-05Licence: In Copyright

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Rogers, G. B., Carroll, M. P., Serisier, D. J., Hockey, P. M., Jones, G., Kehagia, V., Connett, G. J., & Bruce, K. D. (2006). Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis. Journal of Clinical Microbiology, 44(7), 2601-2604. https://doi.org/10.1128/JCM.02282-05

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abstract = "The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.",

keywords = "bacterial community, sputum samples, cystic fibrosis",

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Rogers, GB, Carroll, MP, Serisier, DJ, Hockey, PM, Jones, G, Kehagia, V, Connett, GJ & Bruce, KD 2006, 'Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis', Journal of Clinical Microbiology, vol. 44, no. 7, pp. 2601-2604. https://doi.org/10.1128/JCM.02282-05

Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis. / Rogers, G B; Carroll, M P; Serisier, D J et al.
In: Journal of Clinical Microbiology, Vol. 44, No. 7, 07.2006, p. 2601-2604.

Research output: Contribution to journal › Article › peer-review

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T1 - Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

AU - Rogers, G B

AU - Carroll, M P

AU - Serisier, D J

AU - Hockey, P M

AU - Jones, G

AU - Kehagia, V

AU - Connett, G J

AU - Bruce, K D

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AB - The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.

KW - bacterial community

KW - sputum samples

KW - cystic fibrosis

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DO - 10.1128/JCM.02282-05

M3 - Article

SN - 0095-1137

VL - 44

SP - 2601

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

Abstract

UN SDGs

Keywords

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