Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids

H Flinn, M Burke, C J Stirling, D J Sherratt

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15 Citations (Scopus)

Abstract

Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.
Original languageEnglish
Pages (from-to)2241-2243
Number of pages3
JournalJournal of Bacteriology
Volume171
Issue number4
DOIs
Publication statusPublished - 1989

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