Utilising bacterial communities associated with digested piggery effluent as a primary food source for the batch culture of Moina australiensis

Sayali Patil, Andrew Ward, Martin Kumar, Andrew Ball

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    In this study, a cladoceran planktonic invertebrate, Moina australiensis was uniquely cultured in two stage digested piggery wastewater and fed associated piggery wastewater bacteria. The viability of M. australiensis cultured in digested piggery wastewater under closed dark conditions to limit phytoplankton activity was tested by determining suitable effluent total ammonia nitrogen (TAN) concentrations. The highest total M. australiensis biomass production 0.94 ± 0.47 g and the rate of population increase (r) 0.15 ± 0.08 was recorded in the 30 mg l-1 TAN concentration treatment. The lowest 'r' values and decreased biomass production was observed with increasing TAN concentration levels. This study, also focused on profiling and quantification of the associated bacterial populations in the wastewater culture media and within the digestive tract of M. australiensis by denaturing gradient gel electrophoresis (DGGE) and real-time polymerase chain reaction (RT-PCR) which revealed the feeding specificity of M. australiensis towards "γ-Proteobacteria.". Crown

    Original languageEnglish
    Pages (from-to)3371-3378
    Number of pages8
    JournalBioresource Technology
    Volume101
    Issue number10
    DOIs
    Publication statusPublished - 1 May 2010

    Keywords

    • Denaturing gradient gel electrophoresis (DGGE)
    • Feed specificity
    • Moina australiensis
    • Piggery effluent
    • Real-time polymerase chain reaction (RT-PCR)

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