Utility of peripheral blood B cell subsets analysis in common variable immunodeficiency

M Al Kindi, J Mundy, Thomas Sullivan, William Smith, Frank Kette, Anthony Smith, Robert Heddle, Pravin Hissaria

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    Abnormalities in peripheral blood B cell subsets have been identified in common variable immunodeficiency (CVID) patients and classification systems based upon their numbers have been proposed to predict the clinical features. We analysed B lymphocyte subsets by multi-colour flow cytometry (MFC) in a cohort of well-characterized CVID patients to look at their clinical relevance and validate the published association of different classification criteria (Freiburg, Paris and Euroclass) with clinical manifestations. CVID patients had a reduced proportion of total and switched memory B cells (MBC, swMBC) compared to normal controls (P<0·0006). Patients classified in Freiburg Ia had a higher prevalence of granulomatous diseases (P=0·0034). The previously published associations with autoimmune diseases could not be confirmed. The Euroclass classification was not predictive of clinical phenotypes. The absolute numbers of all B cell subsets were reduced in CVID patients compared to controls. There was a significant linear correlation between low absolute total B cells and MBC with granulomatous disease (P<0·05) and a trend towards lower B cells in patients with autoimmune diseases (P=0·07). Absolute number of different B cell subsets may be more meaningful than their relative percentages in assessing the risk of granulomatous diseases and possibly autoimmunity.

    Original languageEnglish
    Pages (from-to)275-281
    Number of pages7
    JournalClinical and Experimental Immunology
    Issue number2
    Publication statusPublished - Feb 2012


    • Antibody deficiency
    • B cell subtypes
    • Common variable immunodeficiency
    • Flow cytometry
    • Memory B cells

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    Al Kindi, M., Mundy, J., Sullivan, T., Smith, W., Kette, F., Smith, A., Heddle, R., & Hissaria, P. (2012). Utility of peripheral blood B cell subsets analysis in common variable immunodeficiency. Clinical and Experimental Immunology, 167(2), 275-281. https://doi.org/10.1111/j.1365-2249.2011.04507.x