Utility of the HEp-2000 antinuclear antibody substrate

Adrian Y.S. Lee, Dimitra Beroukas, Peter J. Roberts-Thomson

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8 Citations (Scopus)


We write with regards to the recently published consensus paper on antinuclear antibody (ANA) staining patterns by Damoiseaux et al which was most informative.1 We were surprised, however, that there was no mention or discussion of the HEp-2000 substrate (Immunoconcepts, Sacremento, California, USA) despite the acknowledgement that anti-SS-A/Ro60 is frequently missed on indirect immunofluorescence assay (IIFA).1 The HEp-2000 substrate is a modified HEp-2 substrate that is transfected with Ro60 cDNA and hence, over-expresses this antigen.2 It produces a bright nuclear/nucleolar staining pattern under IIFA when anti-SS-A/Ro60 antibodies are present (figure 1), and is one of the most commonly detected extractable nuclear antigen antibodies.3
Original languageEnglish
Pages (from-to)E67
Number of pages1
JournalAnnals of the Rheumatic Diseases
Issue number6
Publication statusPublished - 1 Jun 2020
Externally publishedYes


  • autoantibodies
  • autoimmune diseases
  • autoimmunity
  • HEp-2000


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