Utility of the HEp-2000 antinuclear antibody substrate

Adrian Y.S. Lee; Dimitra Beroukas; Peter J. Roberts-Thomson

doi:10.1136/annrheumdis-2019-215519

Utility of the HEp-2000 antinuclear antibody substrate

Adrian Y.S. Lee, Dimitra Beroukas, Peter J. Roberts-Thomson

Research output: Contribution to journal › Letter › peer-review

10 Citations (Scopus)

Abstract

We write with regards to the recently published consensus paper on antinuclear antibody (ANA) staining patterns by Damoiseaux et al which was most informative.1 We were surprised, however, that there was no mention or discussion of the HEp-2000 substrate (Immunoconcepts, Sacremento, California, USA) despite the acknowledgement that anti-SS-A/Ro60 is frequently missed on indirect immunofluorescence assay (IIFA).1 The HEp-2000 substrate is a modified HEp-2 substrate that is transfected with Ro60 cDNA and hence, over-expresses this antigen.2 It produces a bright nuclear/nucleolar staining pattern under IIFA when anti-SS-A/Ro60 antibodies are present (figure 1), and is one of the most commonly detected extractable nuclear antigen antibodies.3

Original language	English
Pages (from-to)	E67
Number of pages	1
Journal	Annals of the Rheumatic Diseases
Volume	79
Issue number	6
DOIs	https://doi.org/10.1136/annrheumdis-2019-215519
Publication status	Published - 1 Jun 2020
Externally published	Yes

Keywords

autoantibodies
autoimmune diseases
autoimmunity
HEp-2000

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title = "Utility of the HEp-2000 antinuclear antibody substrate",

abstract = "We write with regards to the recently published consensus paper on antinuclear antibody (ANA) staining patterns by Damoiseaux et al which was most informative.1 We were surprised, however, that there was no mention or discussion of the HEp-2000 substrate (Immunoconcepts, Sacremento, California, USA) despite the acknowledgement that anti-SS-A/Ro60 is frequently missed on indirect immunofluorescence assay (IIFA).1 The HEp-2000 substrate is a modified HEp-2 substrate that is transfected with Ro60 cDNA and hence, over-expresses this antigen.2 It produces a bright nuclear/nucleolar staining pattern under IIFA when anti-SS-A/Ro60 antibodies are present (figure 1), and is one of the most commonly detected extractable nuclear antigen antibodies.3",

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T1 - Utility of the HEp-2000 antinuclear antibody substrate

AU - Lee, Adrian Y.S.

AU - Beroukas, Dimitra

AU - Roberts-Thomson, Peter J.

PY - 2020/6/1

Y1 - 2020/6/1

N2 - We write with regards to the recently published consensus paper on antinuclear antibody (ANA) staining patterns by Damoiseaux et al which was most informative.1 We were surprised, however, that there was no mention or discussion of the HEp-2000 substrate (Immunoconcepts, Sacremento, California, USA) despite the acknowledgement that anti-SS-A/Ro60 is frequently missed on indirect immunofluorescence assay (IIFA).1 The HEp-2000 substrate is a modified HEp-2 substrate that is transfected with Ro60 cDNA and hence, over-expresses this antigen.2 It produces a bright nuclear/nucleolar staining pattern under IIFA when anti-SS-A/Ro60 antibodies are present (figure 1), and is one of the most commonly detected extractable nuclear antigen antibodies.3

AB - We write with regards to the recently published consensus paper on antinuclear antibody (ANA) staining patterns by Damoiseaux et al which was most informative.1 We were surprised, however, that there was no mention or discussion of the HEp-2000 substrate (Immunoconcepts, Sacremento, California, USA) despite the acknowledgement that anti-SS-A/Ro60 is frequently missed on indirect immunofluorescence assay (IIFA).1 The HEp-2000 substrate is a modified HEp-2 substrate that is transfected with Ro60 cDNA and hence, over-expresses this antigen.2 It produces a bright nuclear/nucleolar staining pattern under IIFA when anti-SS-A/Ro60 antibodies are present (figure 1), and is one of the most commonly detected extractable nuclear antigen antibodies.3

KW - autoantibodies

KW - autoimmune diseases

KW - autoimmunity

KW - HEp-2000

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SP - E67

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

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Utility of the HEp-2000 antinuclear antibody substrate

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