Utility of the HEp-2000 antinuclear antibody substrate

Adrian Y.S. Lee, Dimitra Beroukas, Peter J. Roberts-Thomson

Research output: Contribution to journalLetter

4 Citations (Scopus)

Abstract

We write with regards to the recently published consensus paper on antinuclear antibody (ANA) staining patterns by Damoiseaux et al which was most informative.1 We were surprised, however, that there was no mention or discussion of the HEp-2000 substrate (Immunoconcepts, Sacremento, California, USA) despite the acknowledgement that anti-SS-A/Ro60 is frequently missed on indirect immunofluorescence assay (IIFA).1 The HEp-2000 substrate is a modified HEp-2 substrate that is transfected with Ro60 cDNA and hence, over-expresses this antigen.2 It produces a bright nuclear/nucleolar staining pattern under IIFA when anti-SS-A/Ro60 antibodies are present (figure 1), and is one of the most commonly detected extractable nuclear antigen antibodies.3
Original languageEnglish
Pages (from-to)E67
Number of pages1
JournalAnnals of the Rheumatic Diseases
Volume79
Issue number6
DOIs
Publication statusPublished - 14 May 2019
Externally publishedYes

Keywords

  • autoantibodies
  • autoimmune diseases
  • autoimmunity
  • HEp-2000

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