Visualisation of catecholamine-fluorescent nerve cell bodies in the rat brain after colchicine treatment

P. R.C. Howe, P. F. Rogers, W. W. Blessing

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    8 Citations (Scopus)

    Abstract

    Central administration of colchicine causes accumulation of catecholamines in nerve cell bodies and enhances their visualisation in the rat brain by the formaldehyde-glutaraldehyde (FAGLU) histofluorescence method. The detection of catecholamine-containing nerve cells by the FAGLU method was considerably improved in rats pretreated with colchicine, and adrenaline-synthesising nerve cells, which are not normally detected by the FAGLU method, could be readily localized after colchicine. The identity of the FAGLU-fluorescent nerve cells was confirmed by simultaneous immunofluorescent detection of catecholamine-synthesising enzymes in sections of the medulla oblongata. The FAGLU method applied to colchicine-treated animals could provide a simple means of detecting central adrenaline nerve cells in other species.

    Original languageEnglish
    Pages (from-to)287-292
    Number of pages6
    JournalNeuroscience letters
    Volume52
    Issue number3
    DOIs
    Publication statusPublished - 21 Dec 1984

    Keywords

    • catecholamines
    • formaldehyde-glutaraldehyde fluorescence
    • immunofluorescence
    • neuroanatomy
    • phenylethanolamine-N-methyl transferase
    • tyrosine hydroxylase

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