Visualisation of catecholamine-fluorescent nerve cell bodies in the rat brain after colchicine treatment

P. R.C. Howe; P. F. Rogers; W. W. Blessing

doi:10.1016/0304-3940(84)90176-9

Visualisation of catecholamine-fluorescent nerve cell bodies in the rat brain after colchicine treatment

P. R.C. Howe, P. F. Rogers, W. W. Blessing

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

Central administration of colchicine causes accumulation of catecholamines in nerve cell bodies and enhances their visualisation in the rat brain by the formaldehyde-glutaraldehyde (FAGLU) histofluorescence method. The detection of catecholamine-containing nerve cells by the FAGLU method was considerably improved in rats pretreated with colchicine, and adrenaline-synthesising nerve cells, which are not normally detected by the FAGLU method, could be readily localized after colchicine. The identity of the FAGLU-fluorescent nerve cells was confirmed by simultaneous immunofluorescent detection of catecholamine-synthesising enzymes in sections of the medulla oblongata. The FAGLU method applied to colchicine-treated animals could provide a simple means of detecting central adrenaline nerve cells in other species.

Original language	English
Pages (from-to)	287-292
Number of pages	6
Journal	Neuroscience letters
Volume	52
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(84)90176-9
Publication status	Published - 21 Dec 1984

Keywords

catecholamines
formaldehyde-glutaraldehyde fluorescence
immunofluorescence
neuroanatomy
phenylethanolamine-N-methyl transferase
tyrosine hydroxylase

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Howe, P. R. C., Rogers, P. F., & Blessing, W. W. (1984). Visualisation of catecholamine-fluorescent nerve cell bodies in the rat brain after colchicine treatment. Neuroscience letters, 52(3), 287-292. https://doi.org/10.1016/0304-3940(84)90176-9

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abstract = "Central administration of colchicine causes accumulation of catecholamines in nerve cell bodies and enhances their visualisation in the rat brain by the formaldehyde-glutaraldehyde (FAGLU) histofluorescence method. The detection of catecholamine-containing nerve cells by the FAGLU method was considerably improved in rats pretreated with colchicine, and adrenaline-synthesising nerve cells, which are not normally detected by the FAGLU method, could be readily localized after colchicine. The identity of the FAGLU-fluorescent nerve cells was confirmed by simultaneous immunofluorescent detection of catecholamine-synthesising enzymes in sections of the medulla oblongata. The FAGLU method applied to colchicine-treated animals could provide a simple means of detecting central adrenaline nerve cells in other species.",

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