Vortex Fluidic Mediated Oxidative Sulfitolysis of Oxytocin

Emily M. Crawley, Scott Pye, Briony E. Forbes, Colin L. Raston

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In peptide production, oxidative sulfitolysis can be used to protect the cysteine residues during purification, and the introduction of a negative charge aids solubility. Subsequent controlled reduction aids in ensuring correct disulfide bridging. In vivo, these problems are overcome through interaction with chaperones. Here, a versatile peptide production process has been developed using an angled vortex fluidic device (VFD), which expands the viable pH range of oxidative sulfitolysis from pH 10.5 under batch conditions, to full conversion within 20 min at pH 9–10.5 utilising the VFD. VFD processing gave 10-fold greater conversion than using traditional batch processing, which has potential in many applications of the sulfitolysis reaction.

Original languageEnglish
Article number1109
Number of pages9
JournalMolecules
Volume27
Issue number3
DOIs
Publication statusPublished - Feb 2022

Keywords

  • Cysteine
  • Peptides
  • Sulfitolysis
  • Vortex fludics

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