Walking, Cloning, and Mapping with YACs in 3q27: Localization of Five ESTs Including Three Members of the Cystatin Gene Family and Identification of CpG Islands

Louise A James, Donald J Ogilvie, Kazuhiro Yamakawa, Yusuke Nakamura, Colin J Stirling, Rakesh Anand

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Using yeast artificial chromosomes, we have generated a high-resolution physical map for 2.7 Mb of human chromosomal region 3q27. The YAC clones group into three contigs, one of which has also been linked to the CEPH YAC contig map of human chromosome 3. Fluorescence in sity hybridization has been used to order the contigs on the chromosome and to estimate the distance between them. Expressed sequence tags for five genes, including three members of the cystatin gene family and a gene thought to be involved in B-cell non-Hodgkin lymphoma, have been placed within the YAC contigs, and 12 putative CpG islands have been identified. These YACs provide a useful resource to complete the physical mapping of 3q27 and to begin identification and characterization of further genes that are located there.
Original languageEnglish
Pages (from-to)425-430
Number of pages6
JournalGenomics
Volume32
Issue number3
DOIs
Publication statusPublished - 1996

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