Wharton's jelly mesenchymal stromal/stem cells derived under chemically defined animal product-free low oxygen conditions are rich in MSCA-1+ subpopulation

Liani Devito, Heba Badraiq, Antonio Galleu, Dheraj K. Taheem, Stefano Codognotto, Richard Siow, Yacoub Khalaf, Annette Briley, Andrew Shennan, Lucilla Poston, John McGrath, Eileen Gentleman, Francesco Dazzi, Dusko Ilic

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Aim: Umbilical cord contains, within Wharton's jelly (WJ), multipotent mesenchymal stromal/stem cells (MSCs) of fetal origin that can be isolated and expanded in vitro with a minimal manipulation and very high efficiency. Our aim was to develop a highly reproducible protocol that has the unique potential to be scaled up and adapted to cGMP requirements for the use in cellular therapy.

Results: We found that derivation of WJ MSCs under defined conditions in low oxygen resulted in several folds higher populations of MSCA-1+ cells (6.0-19.2%) when compared with WJ MSCs derived in the presence of serum (0.1-2.8%) or clinical-grade bone marrow (BM) MSCs cultured under atmospheric O2 (20%). We demonstrate that WJ MSCs derived following our protocol display antiproliferative activity similar to clinical-grade BM MSCs. We also show that these WJ MSCs can be differentiated into adipo-, chondro- and osteo-genic lineages.

Conclusion: Easy accessibility, abundance and genetic 'naivety' make WJ MSCs logistically a more attractive source for clinical applications than BM MSCs.

Original languageEnglish
Pages (from-to)723-732
Number of pages10
JournalRegenerative Medicine
Volume9
Issue number6
DOIs
Publication statusPublished - Nov 2014
Externally publishedYes

Keywords

  • animal product-free culture
  • chemically defined condition
  • mesenchymal stem cell
  • mesenchymal stromal cell
  • MSCA-1
  • umbilical cord
  • Wharton's jelly

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