Where does this tiger come from?-A robust molecular technique for simultaneous identification of endangered species and subspecies

Thitika Kitpipit; Shanan Tobe; Andrew Kitchener; Peter Gill; Adrian Linacre

doi:10.1016/j.fsigss.2011.10.011

Where does this tiger come from?-A robust molecular technique for simultaneous identification of endangered species and subspecies

Thitika Kitpipit, Shanan Tobe, Andrew Kitchener, Peter Gill, Adrian Linacre

Research output: Contribution to conference › Paper › peer-review

1 Citation (Scopus)

Abstract

The Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) monitors the international trade in endangered animal and plant species; a high profile example is the tiger, Panthera tigris. We report on the application of a SNaPshot multiplex technique to simultaneously identify tiger species and subspecies; this test is based on identification of SNPs within the tiger mitochondrial genome. Mitochondrial DNA sequences from four of the five extant putative tiger subspecies were obtained and combined with DNA sequence data from 492 tiger and 349 other mammalian species. A total of 11 SNP loci were identified: five specific for tiger; three specific to Panthera tigris sumatrae and; three specific to P. t. tigris. The multiplex assay was able to reliably identify 15 voucher tiger samples. The sensitivity of the test was 15,000 mitochondrial DNA copies, indicating that it will work on trace amounts of tissue, bone or hair.

Original language	English
Pages	e532-e533
DOIs	https://doi.org/10.1016/j.fsigss.2011.10.011
Publication status	Published - Dec 2011
Event	24th International Congress of the International Society for Forensic Genetics - Duration: 30 Aug 2011 → …

Conference

Conference	24th International Congress of the International Society for Forensic Genetics
Period	30/08/11 → …

Keywords

CITES
Mitochondrial DNA
SNaPshot
SNP
Subspecies
Tiger

Access to Document

10.1016/j.fsigss.2011.10.011

Cite this

@conference{1feb49ea933a4bd489ab1562bfd5a357,

title = "Where does this tiger come from?-A robust molecular technique for simultaneous identification of endangered species and subspecies",

abstract = "The Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) monitors the international trade in endangered animal and plant species; a high profile example is the tiger, Panthera tigris. We report on the application of a SNaPshot multiplex technique to simultaneously identify tiger species and subspecies; this test is based on identification of SNPs within the tiger mitochondrial genome. Mitochondrial DNA sequences from four of the five extant putative tiger subspecies were obtained and combined with DNA sequence data from 492 tiger and 349 other mammalian species. A total of 11 SNP loci were identified: five specific for tiger; three specific to Panthera tigris sumatrae and; three specific to P. t. tigris. The multiplex assay was able to reliably identify 15 voucher tiger samples. The sensitivity of the test was 15,000 mitochondrial DNA copies, indicating that it will work on trace amounts of tissue, bone or hair.",

keywords = "CITES, Mitochondrial DNA, SNaPshot, SNP, Subspecies, Tiger",

author = "Thitika Kitpipit and Shanan Tobe and Andrew Kitchener and Peter Gill and Adrian Linacre",

year = "2011",

month = dec,

doi = "10.1016/j.fsigss.2011.10.011",

language = "English",

pages = "e532--e533",

note = "24th International Congress of the International Society for Forensic Genetics ; Conference date: 30-08-2011",

}

Where does this tiger come from?-A robust molecular technique for simultaneous identification of endangered species and subspecies. / Kitpipit, Thitika; Tobe, Shanan; Kitchener, Andrew et al.
2011. e532-e533 Paper presented at 24th International Congress of the International Society for Forensic Genetics.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Where does this tiger come from?-A robust molecular technique for simultaneous identification of endangered species and subspecies

AU - Kitpipit, Thitika

AU - Tobe, Shanan

AU - Kitchener, Andrew

AU - Gill, Peter

AU - Linacre, Adrian

PY - 2011/12

Y1 - 2011/12

N2 - The Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) monitors the international trade in endangered animal and plant species; a high profile example is the tiger, Panthera tigris. We report on the application of a SNaPshot multiplex technique to simultaneously identify tiger species and subspecies; this test is based on identification of SNPs within the tiger mitochondrial genome. Mitochondrial DNA sequences from four of the five extant putative tiger subspecies were obtained and combined with DNA sequence data from 492 tiger and 349 other mammalian species. A total of 11 SNP loci were identified: five specific for tiger; three specific to Panthera tigris sumatrae and; three specific to P. t. tigris. The multiplex assay was able to reliably identify 15 voucher tiger samples. The sensitivity of the test was 15,000 mitochondrial DNA copies, indicating that it will work on trace amounts of tissue, bone or hair.

AB - The Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) monitors the international trade in endangered animal and plant species; a high profile example is the tiger, Panthera tigris. We report on the application of a SNaPshot multiplex technique to simultaneously identify tiger species and subspecies; this test is based on identification of SNPs within the tiger mitochondrial genome. Mitochondrial DNA sequences from four of the five extant putative tiger subspecies were obtained and combined with DNA sequence data from 492 tiger and 349 other mammalian species. A total of 11 SNP loci were identified: five specific for tiger; three specific to Panthera tigris sumatrae and; three specific to P. t. tigris. The multiplex assay was able to reliably identify 15 voucher tiger samples. The sensitivity of the test was 15,000 mitochondrial DNA copies, indicating that it will work on trace amounts of tissue, bone or hair.

KW - CITES

KW - Mitochondrial DNA

KW - SNaPshot

KW - SNP

KW - Subspecies

KW - Tiger

UR - http://www.scopus.com/inward/record.url?scp=82455212981&partnerID=8YFLogxK

U2 - 10.1016/j.fsigss.2011.10.011

DO - 10.1016/j.fsigss.2011.10.011

M3 - Paper

SP - e532-e533

T2 - 24th International Congress of the International Society for Forensic Genetics

Y2 - 30 August 2011

ER -

Where does this tiger come from?-A robust molecular technique for simultaneous identification of endangered species and subspecies

Abstract

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this