Whole-genome CRISPR screens to understand Apicomplexan-host interactions

Eva Hesping; Justin A. Boddey

doi:10.1111/mmi.15221

Whole-genome CRISPR screens to understand Apicomplexan-host interactions

Eva Hesping, Justin A. Boddey

Research output: Contribution to journal › Review article › peer-review

3 Citations (Scopus)

58 Downloads (Pure)

Abstract

Apicomplexan parasites are aetiological agents of numerous diseases in humans and livestock. Functional genomics studies in these parasites enable the identification of biological mechanisms and protein functions that can be targeted for therapeutic intervention. Recent improvements in forward genetics and whole-genome screens utilising CRISPR/Cas technology have revolutionised the functional analysis of genes during Apicomplexan infection of host cells. Here, we highlight key discoveries from CRISPR/Cas9 screens in Apicomplexa or their infected host cells and discuss remaining challenges to maximise this technology that may help answer fundamental questions about parasite–host interactions.

Original language	English
Pages (from-to)	717-726
Number of pages	10
Journal	MOLECULAR MICROBIOLOGY
Volume	121
Issue number	4
DOIs	https://doi.org/10.1111/mmi.15221
Publication status	Published - Apr 2024
Externally published	Yes

Keywords

Apicomplexa
CRISPR
Cas9
forward genetics
host
parasite

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10.1111/mmi.15221Licence: CC BY

Final published versionFinal published version, 3.53 MBLicence: CC BY

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AB - Apicomplexan parasites are aetiological agents of numerous diseases in humans and livestock. Functional genomics studies in these parasites enable the identification of biological mechanisms and protein functions that can be targeted for therapeutic intervention. Recent improvements in forward genetics and whole-genome screens utilising CRISPR/Cas technology have revolutionised the functional analysis of genes during Apicomplexan infection of host cells. Here, we highlight key discoveries from CRISPR/Cas9 screens in Apicomplexa or their infected host cells and discuss remaining challenges to maximise this technology that may help answer fundamental questions about parasite–host interactions.

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KW - CRISPR

KW - Cas9

KW - forward genetics

KW - host

KW - parasite

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Whole-genome CRISPR screens to understand Apicomplexan-host interactions

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Keywords

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